Aim: To identify
immunodominant proteins of cystic fluid antigens and whole cyst lysate antigens
of Cysticercus tenuicollis, the larval stage of the canine tapeworm Taenia
hydatigena.
Materials and Methods: Three numbers of cysts of C.
tenuicollis were collected from the mesentry of small intestine of goats after
slaughter. C. tenuicollis cysts of each sample were washed thoroughly with PBS
(pH 7.4). Two types of antigens i.e. cystic fluid antigens and whole cyst lysate
antigens were prepared from each sample. Polypeptide profiles of cystic fluid
antigens and whole cyst lysate antigens preparations of C. tunicollis were
analysed by Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)
containing 10% gel. C. tenuicollis proteins resolved by SDS-PAGE were
electrophoretically transferred from the gel to a nitrocellulose membrane and
probed by western blotting using natural immune sera obtained from infected
goats. C. tenuicollis proteins that were resolved by SDS-PAGE and reactive by
immune sera of goat was estimated by a Soft ware programme named KODAK 1D Image
Analysis Software.
Results: A total of eight major polypeptides of
molecular weight (Mr) 149.4 kDa, 92.9 kDa, 74.2 kDa, 63.5 kDa, 36.2 kDa, 23.9
kDa, 15.7 kDa and 9.6 kDa were resolved by SDS-PAGE with minor variations. Out
of these, three polypeptides of Mr 36.2 kDa, 23.9 kDa and 9.6 kDa were
recognized as immunodominant polypeptides after western blotting. Both cystic
fluid antigens and whole cyst lysate antigens resolved same Mr polypeptides by
SDS-PAGE and identified same immunodominant polypeptides after western blotting.
The immunodominant polipeptides of Mr 23.9 kDa and 9.6 kDa identified for the
first time from both cystic fluid antigens and whole cyst lysate antigens
prepared from Cysticercus tenuicollis.
Conclusion: Eight polypeptides
were resolved from cystic fluid antigens and whole cyst lysate antigens of
Cysticercus tenuicollis by SDS-PAGe of which polypeptides of three polypeptides
of Mr 36.2 kDa, 23.9 kDa and 9.6 kDa were recognized as immunodominant
polypeptides after western blotting. These three immunodominant polypeptides
could be explored for sero diagnosis of C. tenuicollis infection in goats.
Keywords: Cysticercus tenuicollis, goat, immunogenic, polypeptide
